Differential media allow the characterization of several microorganisms based on the growth patterns of them. Example of selective media added with chemicals: The most common growth media for microorganisms are nutrient broths (liquid nutrient medium) or lysogeny broth medium. MacConkey agar for Gram-negative bacteria. MANNITOL SALT AGAR (MSA) Mannitol salt agar is a selective medium used for the isolation of pathogenic staphylococci. Understand how the different bacteria grow on these media. Selective: high salt selects for Staphylococci. These media favour the growth of a particular bacterium by inhibiting the growth of undesired bacteria and allowing growth If so please see the note that follows. Distinguish between selective and differential media. For example, if a microorganism is resistant to a certain antibiotic, such as ampicillin or tetracycline, then that antibiotic can be added to the medium in order to prevent other cells, which do â¦ This gives biologists the ability to control what types of organisms grow in a specific experiment. Potassium tellurite medium used to recover C.diphtheriae contains 0.04% potassium tellurite. Examples include sheep blood agar and chocolate (heated blood) agar. Differential Media: Differential media allow several closely-related microorganisms to grow in the medium. Watch the recordings here on Youtube! The main difference between selective and differential media is their role in the identification of microorganisms. These media are non selective and they contain general nutrients which required for the growth of wide range of microorganisms. Media that inhibit the growth of unwanted microorganisms and support the growth of the organism of interest by supplying nutrients and reducing competition are called selective media. This type of media is used for the detection of microorganisms and by molecular biologists to detect recombinant strains of bacteria. “Culturing Media.” Boundless Microbiology, Available here. Some differential media are also selective, for example, standard enteric agars such as MacConkey and EMB agars, which are selective for gram-negative coliforms and can differentiate lactose-fermenting and non-lactose-fermenting bacteria. They remain solid, as very few bacteria are able to decompose agar (the exception being some species in the genera: Cytophaga, Flavobacterium, Bacillus, Pseudomonas, and Alcaligenes). Functionally, selective media permit certain types of organisms to grow while inhibiting the Non-pathogenic Staphylococci do not ferment mannitol and so leave the media pink. Selective growth media also ensures the survival and proliferation of microorganisms with certain properties. Medium formulated to permit the growth of certain bacteria but not others. Nutrient media: These media are also called general purpose media. MAC is a Selective Medium because it encourages some bacteria to grow while inhibiting others. Selective media are used to isolate a particular type of microorganism by giving a specific condition for the growth of that particular microorganism. The MacConkeyâs and Mannitol Salt described above are both selective and differential bacterial growth media. Selective Media: Selective media use specific growth characteristics of a particular microorganism to select it from the others. These agar plates provide a solid medium on which microbes may be cultured. Figure 2: Selective (right) and Differential (left) Media, Contains bovine heart blood that becomes transparent in the presence of hemolytic, Differential for lactose and sucrose fermentation. Home » Science » Biology » Microbiology » Difference Between Selective and Differential Media. Selective and differential media are two types of media used to isolate and identify microorganisms. Have questions or comments? YM (yeast and mold) which has a low pH, deterring bacterial growth. Selective medium. USE OF SELECTIVE AND DIFFERENTIAL MEDIA Overview Samples obtained from nature are most often a mix of many types of microorganisms, which can be identified or isolated by several methods. What is Differential Media – Definition, Role, Examples 3. MacConkey Agar (or MAC) is a Selective & Differential Medium. Salt Milk agar used to identify and differentiate microorganisms from a closely-related group with the help of unique patterns! 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